| 1 |
Course Overview, Enzyme Classification |
|
| 2 |
Enzyme Catalysts: Review of Protein Chemistry, Enzyme Specificity |
|
| 3-4 |
Lecture 3
Non-kinetic Evaluation of Enzyme Performance, Review of Enzyme Kinetics
Lecture 4
Enzyme Kinetics (cont.), pH and Temperature Effects, Enzyme Stability |
|
| 5-6 |
Lecture 5
Enzyme Inhibition and Toxicity
Lecture 6
Enzyme Inhibition and Toxicity (cont.) |
Lecture 5
Homework #1 due
|
| 7 |
Whole Cell Catalysts: When/Why, Cell Growth vs. Conversion |
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| 8 |
Control of Protein Expression (Growth/Non-growth Associated, Catabolite Repression)
Toxicity and Inhibition |
|
| 9-11 |
Lecture 9
Engineering Solutions: Acidic/Basic Products, Exothermic Reactions, Enzyme Instability, Equilibrium Reactions
Lecture 10
Substrate Solubility, Toxicity
Lecture 11
Product Solubility, Toxicity
Yield Improvement of Resolutions |
Lecture 9
Homework #2 due
Lecture 11
Design report distributed
|
| 12-14 |
Lecture 12
Process Design for Biocatalytic Processes: Purified Enzymes
Lecture 13
Process Design (cont.): Whole Cells
Lecture 14
Screening for New Catalysts |
|
| 15-17 |
Lecture 15
Improving Catalyst Performance (Biological Solutions): Review of Molecular Biology
Lecture 16
Cloning from Whole Cell Catalysts
Lecture 17
Site-directed Mutagenesis
Directed Evolution of Enzymes |
Lecture 15
Homework #3 due
|
| 18 |
Presentations of Design/Development Reports |
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